EXPERIMENTAL STUDY OF THE WOUND-HEALING PROPERTIES OF A SUBSTANCE BASED ON THE ZHUZGUN PLANT (G15)

Abstract

Introduction. The use of medicinal herbs is becoming increasingly important as an alternative treatment in many countries for the treatment of wounds and burn injuries. Plants from the Buckwheat family contain biologically active substances: flavonoids, tannins, terpenoids, amino acids, carbohydrates, trace elements, essential oils, which determines its use in traditional medicine as an anti-inflammatory agent. Aim of the study. Evaluation of the wound-healing properties of the G15 substance from the Zhuzgun of the Buckwheat family an experimental model of a burn wound in rats. Materials and methods. The substance was provided L.N. Gumilyov Eurasian National University. An ointment was prepared of Lipoderm: 1%, 3% and 5% ointment of substance G15. 10% Methyluracil ointment, Sea Buckthorn oil were taken as comparison drugs. The study of the wound-healing activity of the substance was carried out on burn wound model in rats in 6 groups. The process of tissue regeneration was assessed by the timing of burn scab rejection, the timing of epithelization and complete healing on days 1, 7, 14, 21 and 28. Results of histological analysis on days 1, 15 and 30 of the study. Results. Wound healing in the experimental groups of animals was characterized by an easier course of the process, stopping the spread of necrosis into the deep layers of the skin. In general, the shortest healing time for burn wounds was observed when using 5% and methyluracil ointment. So, in comparison with control, the wound healing time was reduced by 12,0 and 10,72 days, respectively, less pronounced data in other experimental groups. Histomorphological studies confirmed the dynamics of skin tissue restoration after a burn. Conclusions. The data obtained indicate that 5% ointment based on the substance from Zhuzgun has wound-healing activity, is superior in its effectiveness to sea buckthorn oil, 1% and 3% ointment, and is comparable in effectiveness to methyluracil ointment.